|
|
|
|
| |
|
|
| Oliveira,Camila Franco Batista; Alves,Daniela Pereira; Emerich,Bruna Luiza; Figueiredo,Suely Gomes de; Cordeiro,Marta do Nascimento; Borges,Márcia Helena; Richardson,Michael; Pimenta,Adriano Monteiro de Castro; Duarte,Igor Dimitri Gama; Lima,Maria Elena de. |
| ABSTRACT Background: The venom of Phoneutria nigriventer spider is a source of numerous bioactive substances, including some toxins active in insects. An example is PnTx4(5-5) that shows a high insecticidal activity and no apparent toxicity to mice, although it inhibited NMDA-evoked currents in rat hippocampal neurons. In this work the analgesic activity of PnTx4(5-5) (renamed Γ-ctenitoxin-Pn1a) was investigated. Methods: The antinociceptive activity was evaluated using the paw pressure test in rats, after hyperalgesia induction with intraplantar injection of carrageenan or prostaglandin E2 (PGE2). Results: PnTx4(5-5), subcutaneously injected, was able to reduce the hyperalgesia induced by PGE2 in rat paw, demonstrating a systemic effect. PnTx4(5-5)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spider toxin; Γ-ctenitoxin-Pn1a; PnTx4(5-5); Phoneutria nigriventer; Antinociception; Glutamate. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100316 |
| |
|
|
| Santos,R.G.; Diniz,C.R.; Cordeiro,M.N.; De Lima,M.E.. |
| Tx1, a neurotoxin isolated from the venom of the South American spider Phoneutria nigriventer, produces tail elevation, behavioral excitation and spastic paralysis of the hind limbs after intracerebroventricular injection in mice. Since Tx1 contracts isolated guinea pig ileum, we have investigated the effect of this toxin on acetylcholine release, as well as its binding to myenteric plexus-longitudinal muscle membranes from the guinea pig ileum. [125I]-Tx1 binds specifically and with high affinity (Kd = 0.36 ± 0.02 nM) to a single, non-interacting (nH = 1.1), low capacity (Bmax 1.1 pmol/mg protein) binding site. In competition experiments using several compounds (including ion channel ligands), only PhTx2 and PhTx3 competed with [125I]-Tx1 for specific... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Phoneutria nigriventer; Spider toxins; Binding; Enteric nervous system; Calcium channels; Guinea pig ileum. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200019 |
| |
|
|
| Yonamine,C. M.; Costa,H.; Silva,J. A. A.; Muramoto,E.; Rogero,J. R.; Troncone,L. R. P.; Camillo,M. A. P.. |
| The use of radiotracers allows the understanding of the bioavailability process, biodistribution, and kinetics of any molecule labelled with an isotope, which does not alter the molecule's biological properties. In this work, technetium-99m and iodine-125 were chosen as radiotracers for biodistribution studies in mice using bee (Apis mellifera) venom and a toxin (PnTX2-6) from the Brazilian "armed" spider (Phoneutria nigriventer) venom. Incorporated radioactivity was measured in the blood, brain, heart, lung, liver, kidney, adrenal gland, spleen, stomach, testicle, intestine, muscle, and thyroid gland. Results provided the blood kinetic parameter, and different organs distribution rates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phoneutria nigriventer; Apis mellifera; Toxin; Venom; Radiotracer; Biodistribution. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100006 |
| |
|
|
| FERNANDES,H. S.; SAKATE,M.; CHERUBINI,A. L.; PADOVANI,A. F.; MENESES,A. M. C.. |
| This report refers to a female Pinscher treated at the Veterinary Hospital of the Botucatu School of Veterinary Medicine and Animal Husbandry (FMVZ)-São Paulo State University (UNESP), Botucatu, 14 hours after a suspected spider envenoming. The animal showed spastic limbs, dyspnea, salivation, hypothermia, muscular fasciculation, non-responsive mydriasis, and claudication of the right hindlimb. The spider was later identified by the Center for the Study of Venoms and Venomous Animals-CEVAP/UNESP, Botucatu as Phoneutria nigriventer. The treatment with potent analgesic was efficient. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Spider envenoming; Phoneutria nigriventer; Dog. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100014 |
| |
|
|
|
